Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26426 | 5' | -55.1 | NC_005345.2 | + | 23710 | 0.69 | 0.472422 |
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Target: 5'- cGGUACggGGCUCuGCGCUcgcuucCUGGCGGGAa -3' miRNA: 3'- -CCGUG--UCGAG-CGUGAcu----GACUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 16769 | 0.69 | 0.472422 |
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Target: 5'- -aCGCGGUgagCGCACUGuCcGACAGGGu -3' miRNA: 3'- ccGUGUCGa--GCGUGACuGaCUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 33670 | 0.73 | 0.286801 |
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Target: 5'- cGGCGCAGCUCGgGCUcGACccgaaGCAGGc -3' miRNA: 3'- -CCGUGUCGAGCgUGA-CUGac---UGUCCu -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 18979 | 0.73 | 0.279556 |
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Target: 5'- cGCAUGGgUCGcCGCUGGCcGGCGGGAc -3' miRNA: 3'- cCGUGUCgAGC-GUGACUGaCUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 12531 | 0.77 | 0.159123 |
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Target: 5'- cGCGCAGCUCGCGgaGcgggcgcagcGCUGGCAGGu -3' miRNA: 3'- cCGUGUCGAGCGUgaC----------UGACUGUCCu -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 44740 | 1.11 | 0.000593 |
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Target: 5'- uGGCACAGCUCGCACUGACUGACAGGAg -3' miRNA: 3'- -CCGUGUCGAGCGUGACUGACUGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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