Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26427 | 3' | -55.1 | NC_005345.2 | + | 17148 | 0.67 | 0.662386 |
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Target: 5'- aCGUUGGuCGGGGCGgUCGUGccgacgggGGCGGc- -3' miRNA: 3'- -GCAGCC-GCUCUGUgAGCAC--------UCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 29530 | 0.68 | 0.618378 |
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Target: 5'- uCGUCGGCgucGAGGUACUCGgGGGCGu-- -3' miRNA: 3'- -GCAGCCG---CUCUGUGAGCaCUCGCuac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 5585 | 0.7 | 0.479413 |
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Target: 5'- aGUUGGCGAGGCGgUCGgcGAGCu--- -3' miRNA: 3'- gCAGCCGCUCUGUgAGCa-CUCGcuac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 3079 | 0.7 | 0.459286 |
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Target: 5'- uGUCGGCGAgGACACUgG-GAaUGAUGg -3' miRNA: 3'- gCAGCCGCU-CUGUGAgCaCUcGCUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 18936 | 0.71 | 0.439618 |
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Target: 5'- gCGUCGGCGucGACGuugauCUCGUGgcGGCGAg- -3' miRNA: 3'- -GCAGCCGCu-CUGU-----GAGCAC--UCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 23928 | 0.72 | 0.357487 |
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Target: 5'- cCGUCGugcGUGAGcucGCACUCGgcaGAGCGGUGu -3' miRNA: 3'- -GCAGC---CGCUC---UGUGAGCa--CUCGCUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 22690 | 0.72 | 0.349051 |
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Target: 5'- aCGUCGGCGuuuGGCugaUCGUGAGC-AUGu -3' miRNA: 3'- -GCAGCCGCu--CUGug-AGCACUCGcUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 41646 | 0.73 | 0.309022 |
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Target: 5'- gGUCGGCGAGcCACUccagCGUGAGgGGc- -3' miRNA: 3'- gCAGCCGCUCuGUGA----GCACUCgCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 1334 | 0.66 | 0.737882 |
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Target: 5'- gGUCcuccagGGCGAGGCGCUgucgaaGUGGGuUGAUGu -3' miRNA: 3'- gCAG------CCGCUCUGUGAg-----CACUC-GCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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