Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26427 | 3' | -55.1 | NC_005345.2 | + | 43514 | 0.71 | 0.41104 |
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Target: 5'- uCG-CGGCGAacccGGCGCUCGcgGAGCGGa- -3' miRNA: 3'- -GCaGCCGCU----CUGUGAGCa-CUCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 23928 | 0.72 | 0.357487 |
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Target: 5'- cCGUCGugcGUGAGcucGCACUCGgcaGAGCGGUGu -3' miRNA: 3'- -GCAGC---CGCUC---UGUGAGCa--CUCGCUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 22690 | 0.72 | 0.349051 |
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Target: 5'- aCGUCGGCGuuuGGCugaUCGUGAGC-AUGu -3' miRNA: 3'- -GCAGCCGCu--CUGug-AGCACUCGcUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 41646 | 0.73 | 0.309022 |
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Target: 5'- gGUCGGCGAGcCACUccagCGUGAGgGGc- -3' miRNA: 3'- gCAGCCGCUCuGUGA----GCACUCgCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 14328 | 0.74 | 0.301449 |
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Target: 5'- aCGUCGGCGAGgggcaggcGCACggCGUGcGCGAg- -3' miRNA: 3'- -GCAGCCGCUC--------UGUGa-GCACuCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 35843 | 0.74 | 0.27259 |
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Target: 5'- gCG-CGGCGGGugGCUCGguucGGGCGggGg -3' miRNA: 3'- -GCaGCCGCUCugUGAGCa---CUCGCuaC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 43738 | 0.76 | 0.21241 |
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Target: 5'- -cUCGGCGAGACGCUCGgccgaccgccgggccUGAGUGGa- -3' miRNA: 3'- gcAGCCGCUCUGUGAGC---------------ACUCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 18192 | 0.77 | 0.194001 |
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Target: 5'- cCGUCGGCGAGGCGCgCGccGAGUGGg- -3' miRNA: 3'- -GCAGCCGCUCUGUGaGCa-CUCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 45026 | 1.09 | 0.000956 |
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Target: 5'- aCGUCGGCGAGACACUCGUGAGCGAUGa -3' miRNA: 3'- -GCAGCCGCUCUGUGAGCACUCGCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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