Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2643 | 5' | -56.9 | NC_001491.2 | + | 59146 | 0.66 | 0.85586 |
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Target: 5'- gUCAAGCGGUaucucuccagGUGAGCucuaaacgccuCGCGGUGg -3' miRNA: 3'- -AGUUCGUCAgcca------CACUCG-----------GCGCCAC- -5' |
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| 2643 | 5' | -56.9 | NC_001491.2 | + | 20712 | 0.67 | 0.800669 |
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Target: 5'- uUguAGCGGcCGuuGUGgGAGCCGCGGUc -3' miRNA: 3'- -AguUCGUCaGC--CACaCUCGGCGCCAc -5' |
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| 2643 | 5' | -56.9 | NC_001491.2 | + | 20394 | 0.72 | 0.543343 |
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Target: 5'- --uGGUAGccgCGGUGgugGuAGCCGCGGUGg -3' miRNA: 3'- aguUCGUCa--GCCACa--C-UCGGCGCCAC- -5' |
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| 2643 | 5' | -56.9 | NC_001491.2 | + | 20364 | 0.72 | 0.543343 |
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Target: 5'- --uGGUAGccgCGGUGgugGuAGCCGCGGUGg -3' miRNA: 3'- aguUCGUCa--GCCACa--C-UCGGCGCCAC- -5' |
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| 2643 | 5' | -56.9 | NC_001491.2 | + | 20331 | 0.72 | 0.53344 |
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Target: 5'- uUguGGUAGccgCGGUGgugGuAGCCGCGGUGg -3' miRNA: 3'- -AguUCGUCa--GCCACa--C-UCGGCGCCAC- -5' |
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| 2643 | 5' | -56.9 | NC_001491.2 | + | 118318 | 1.08 | 0.002722 |
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Target: 5'- gUCAAGCAGUCGGUGUGAGCCGCGGUGc -3' miRNA: 3'- -AGUUCGUCAGCCACACUCGGCGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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