Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26431 | 3' | -57.7 | NC_005345.2 | + | 21677 | 0.67 | 0.457002 |
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Target: 5'- -aUCCUGGUUCCCgggcagggGCUCGggcuugcagugacagCCGgGGu -3' miRNA: 3'- aaAGGACUAGGGGa-------CGAGCa--------------GGCgCC- -5' |
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| 26431 | 3' | -57.7 | NC_005345.2 | + | 2263 | 0.67 | 0.456016 |
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Target: 5'- --gCCaGAUUCUCggugcGCUCGUCCGCaGGg -3' miRNA: 3'- aaaGGaCUAGGGGa----CGAGCAGGCG-CC- -5' |
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| 26431 | 3' | -57.7 | NC_005345.2 | + | 9440 | 0.68 | 0.408268 |
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Target: 5'- -gUCgUGG-CCCgaGCUCGUCC-CGGg -3' miRNA: 3'- aaAGgACUaGGGgaCGAGCAGGcGCC- -5' |
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| 26431 | 3' | -57.7 | NC_005345.2 | + | 31577 | 0.69 | 0.372397 |
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Target: 5'- --aCCUGAUgCagcucugccgggCCCUGCUCGgcgcggcCCGCGGa -3' miRNA: 3'- aaaGGACUA-G------------GGGACGAGCa------GGCGCC- -5' |
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| 26431 | 3' | -57.7 | NC_005345.2 | + | 28742 | 0.69 | 0.355283 |
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Target: 5'- gUUCCUGuacUCggCCCUGCaggCGcCCGCGGu -3' miRNA: 3'- aAAGGACu--AG--GGGACGa--GCaGGCGCC- -5' |
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| 26431 | 3' | -57.7 | NC_005345.2 | + | 32195 | 0.71 | 0.274029 |
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Target: 5'- --aCCUGGucguguucgggcucgUCaaCCCggGCUCGUCCGCGGu -3' miRNA: 3'- aaaGGACU---------------AG--GGGa-CGAGCAGGCGCC- -5' |
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| 26431 | 3' | -57.7 | NC_005345.2 | + | 18243 | 0.73 | 0.215051 |
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Target: 5'- --cCCUGGUCCCggugCUGCUCGccggCCGCGc -3' miRNA: 3'- aaaGGACUAGGG----GACGAGCa---GGCGCc -5' |
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| 26431 | 3' | -57.7 | NC_005345.2 | + | 48309 | 1.07 | 0.000651 |
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Target: 5'- gUUUCCUGAUCCCCUGCUCGUCCGCGGu -3' miRNA: 3'- -AAAGGACUAGGGGACGAGCAGGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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