Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26432 | 3' | -55.3 | NC_005345.2 | + | 19152 | 0.68 | 0.552416 |
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Target: 5'- -cCGGGAcgucgucgccGGGCCCguacggcugcCCGUCGACGUGCAc -3' miRNA: 3'- auGCUCU----------UCUGGG----------GGCGGCUGUAUGU- -5' |
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| 26432 | 3' | -55.3 | NC_005345.2 | + | 34823 | 0.68 | 0.520038 |
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Target: 5'- gUGCGccu-GcCCCUCGCCGACGUGCu -3' miRNA: 3'- -AUGCucuuCuGGGGGCGGCUGUAUGu -5' |
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| 26432 | 3' | -55.3 | NC_005345.2 | + | 2556 | 0.72 | 0.329997 |
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Target: 5'- gACGGcGAGugCCCgGUCGACGUGCu -3' miRNA: 3'- aUGCUcUUCugGGGgCGGCUGUAUGu -5' |
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| 26432 | 3' | -55.3 | NC_005345.2 | + | 39868 | 0.73 | 0.291199 |
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Target: 5'- cGCGAGGuacGCCgCgGCCGACAUGCGg -3' miRNA: 3'- aUGCUCUuc-UGGgGgCGGCUGUAUGU- -5' |
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| 26432 | 3' | -55.3 | NC_005345.2 | + | 11616 | 0.74 | 0.249468 |
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Target: 5'- gGCGAGggGAUCacagCCCGCCGAgCAgGCAu -3' miRNA: 3'- aUGCUCuuCUGG----GGGCGGCU-GUaUGU- -5' |
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| 26432 | 3' | -55.3 | NC_005345.2 | + | 16632 | 0.74 | 0.243013 |
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Target: 5'- cGCGcAGAucGGGCCgCCCGCCGGCAgagggACGg -3' miRNA: 3'- aUGC-UCU--UCUGG-GGGCGGCUGUa----UGU- -5' |
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| 26432 | 3' | -55.3 | NC_005345.2 | + | 33878 | 0.77 | 0.161997 |
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Target: 5'- aACGAGcucccACCCCCGCCGACGUGa- -3' miRNA: 3'- aUGCUCuuc--UGGGGGCGGCUGUAUgu -5' |
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| 26432 | 3' | -55.3 | NC_005345.2 | + | 12654 | 0.66 | 0.69636 |
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Target: 5'- gGCGcGGucGGCCgCCGuuGGCGUACc -3' miRNA: 3'- aUGC-UCuuCUGGgGGCggCUGUAUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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