Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26432 | 5' | -63.2 | NC_005345.2 | + | 44974 | 0.7 | 0.150643 |
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Target: 5'- cCUGCCgccccGUGaGCGCCuGCCgcgucgacaCCCGCu -3' miRNA: 3'- aGACGGa----CAC-CGCGGuCGGag-------GGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 9304 | 0.7 | 0.142836 |
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Target: 5'- cCUGCCagGUGcG-GCCGGCCgagCCCGCg -3' miRNA: 3'- aGACGGa-CAC-CgCGGUCGGag-GGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 47606 | 0.72 | 0.114563 |
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Target: 5'- gUCUGCUggUGUGGgG-CAGCCguacacauccugCCCCGCg -3' miRNA: 3'- -AGACGG--ACACCgCgGUCGGa-----------GGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 43848 | 0.72 | 0.103312 |
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Target: 5'- aCUGCCcGgucgacggGGCGUCGGCCgggaCUCCGCg -3' miRNA: 3'- aGACGGaCa-------CCGCGGUCGGa---GGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 8005 | 0.73 | 0.097816 |
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Target: 5'- gUCUGCaCaGUGGCgagccGCCAGUCggagCCCCGUa -3' miRNA: 3'- -AGACG-GaCACCG-----CGGUCGGa---GGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 11068 | 0.73 | 0.087643 |
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Target: 5'- aUCUgcGCCUgcGUGGCGCCcggcGGCgcaaUCCCCGCc -3' miRNA: 3'- -AGA--CGGA--CACCGCGG----UCGg---AGGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 43670 | 0.74 | 0.07024 |
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Target: 5'- cCUGCCgccgccggGUGGCuGaUCAGCCUCgCCGCg -3' miRNA: 3'- aGACGGa-------CACCG-C-GGUCGGAGgGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 22358 | 0.75 | 0.066438 |
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Target: 5'- --cGCCUGcgacgcGGCGCCGGCCacgaUCCgCCGCa -3' miRNA: 3'- agaCGGACa-----CCGCGGUCGG----AGG-GGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 48921 | 1.1 | 0.000108 |
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Target: 5'- cUCUGCCUGUGGCGCCAGCCUCCCCGCu -3' miRNA: 3'- -AGACGGACACCGCGGUCGGAGGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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