Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26435 | 5' | -40 | NC_005354.1 | + | 27527 | 0.66 | 0.999998 |
Target: 5'- --aGAUGcuAAGCGACCAAUcuaAGGGcGCUg -3' miRNA: 3'- cgaCUAU--UUCGUUGGUUA---UUUCaUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 12515 | 0.65 | 0.999998 |
Target: 5'- uGCUGAggcaaugguaAAAGCGACUAGUGA--UGCa -3' miRNA: 3'- -CGACUa---------UUUCGUUGGUUAUUucAUGg -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 8967 | 0.66 | 0.999998 |
Target: 5'- --aGAUAGGGCuguuAGCUGAUcgguAGUACCa -3' miRNA: 3'- cgaCUAUUUCG----UUGGUUAuu--UCAUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 37675 | 0.66 | 0.999994 |
Target: 5'- aCUGGUGgcGCAAUUAAcuuacgcUGGGGUGCUa -3' miRNA: 3'- cGACUAUuuCGUUGGUU-------AUUUCAUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 5884 | 0.66 | 0.999994 |
Target: 5'- aGCUGA---GGU-ACCAAaucAGUACCg -3' miRNA: 3'- -CGACUauuUCGuUGGUUauuUCAUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 4309 | 0.66 | 0.999994 |
Target: 5'- uCUGAUAAAGCAAaaGAUAGA--ACUu -3' miRNA: 3'- cGACUAUUUCGUUggUUAUUUcaUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 21783 | 0.68 | 0.999894 |
Target: 5'- aCUGAUGGuGCAaaGCCGAcUGAAGaUGCUa -3' miRNA: 3'- cGACUAUUuCGU--UGGUU-AUUUC-AUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 25497 | 0.7 | 0.999549 |
Target: 5'- aUUGGUAcAGCAguaGCCAauAUGAAGaACCg -3' miRNA: 3'- cGACUAUuUCGU---UGGU--UAUUUCaUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 27142 | 0.71 | 0.99844 |
Target: 5'- uCUGAUAuugguAAGCAauggcagaacuuaGCUAAUAAuAGUGCCa -3' miRNA: 3'- cGACUAU-----UUCGU-------------UGGUUAUU-UCAUGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 10552 | 0.72 | 0.995745 |
Target: 5'- uGUUcGUAAGGCAACUGAuUGGAGUACa -3' miRNA: 3'- -CGAcUAUUUCGUUGGUU-AUUUCAUGg -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 33075 | 0.74 | 0.986095 |
Target: 5'- gGCUGAUAAuGGCAaaauuuuGCCAGUAcAGUgguaugACCa -3' miRNA: 3'- -CGACUAUU-UCGU-------UGGUUAUuUCA------UGG- -5' |
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26435 | 5' | -40 | NC_005354.1 | + | 26981 | 1.21 | 0.006599 |
Target: 5'- cGCUGAUAAAGCAACCAAUAAAGUACCa -3' miRNA: 3'- -CGACUAUUUCGUUGGUUAUUUCAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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