Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2644 | 5' | -59.7 | NC_001491.2 | + | 8450 | 0.66 | 0.711657 |
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Target: 5'- cGUGCGAcggauccUGCcGCau-GGCCCaGCGGGg -3' miRNA: 3'- -UACGCU-------ACGaCGcauCCGGGcCGUCC- -5' |
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| 2644 | 5' | -59.7 | NC_001491.2 | + | 91403 | 0.67 | 0.66178 |
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Target: 5'- -gGCcaGGUGCUGCGUgcGGGCUcccaaagCGuGCAGGa -3' miRNA: 3'- uaCG--CUACGACGCA--UCCGG-------GC-CGUCC- -5' |
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| 2644 | 5' | -59.7 | NC_001491.2 | + | 3014 | 0.69 | 0.562208 |
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Target: 5'- -gGCGuuUGuCUGgGU-GGCCCGGCAGc -3' miRNA: 3'- uaCGCu-AC-GACgCAuCCGGGCCGUCc -5' |
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| 2644 | 5' | -59.7 | NC_001491.2 | + | 11475 | 0.69 | 0.54245 |
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Target: 5'- -cGCGcgGCUGCGgggcugccuGGCCCcucugGGguGGg -3' miRNA: 3'- uaCGCuaCGACGCau-------CCGGG-----CCguCC- -5' |
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| 2644 | 5' | -59.7 | NC_001491.2 | + | 69110 | 0.73 | 0.347752 |
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Target: 5'- aGUGCGGcgGCUGCGUAGGC--GGCGGu -3' miRNA: 3'- -UACGCUa-CGACGCAUCCGggCCGUCc -5' |
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| 2644 | 5' | -59.7 | NC_001491.2 | + | 116592 | 1.07 | 0.001576 |
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Target: 5'- uAUGCGAUGCUGCGUAGGCCCGGCAGGc -3' miRNA: 3'- -UACGCUACGACGCAUCCGGGCCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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