Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26442 | 3' | -56.3 | NC_005357.1 | + | 13992 | 0.66 | 0.571205 |
Target: 5'- cGCUGGCCUcgUGCUGcaCAGCUUGa--- -3' miRNA: 3'- -CGACCGGG--ACGACaaGUUGAGCcgag -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 14268 | 0.66 | 0.5601 |
Target: 5'- gGUUGGCCUgcugcaccaUGCUG-UCGGCgaugCGGCcCa -3' miRNA: 3'- -CGACCGGG---------ACGACaAGUUGa---GCCGaG- -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 33594 | 0.66 | 0.5601 |
Target: 5'- cGCUGGCCCgcguugcgGCUGgccCGACccagcagaUCGGUa- -3' miRNA: 3'- -CGACCGGGa-------CGACaa-GUUG--------AGCCGag -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 38384 | 0.66 | 0.52718 |
Target: 5'- cGCUGGCCUgccGCUcgccgCGGC-CGGCUUu -3' miRNA: 3'- -CGACCGGGa--CGAcaa--GUUGaGCCGAG- -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 23016 | 0.67 | 0.495011 |
Target: 5'- uGCUGcuGCgCCUGCUGUUgGGCUUGuuGCUg -3' miRNA: 3'- -CGAC--CG-GGACGACAAgUUGAGC--CGAg -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 28098 | 0.67 | 0.495011 |
Target: 5'- aGCaGGCCCgGCaGUUCAGCcaaGGCg- -3' miRNA: 3'- -CGaCCGGGaCGaCAAGUUGag-CCGag -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 31520 | 0.67 | 0.490788 |
Target: 5'- uGCUGGgCCUcGCUGUguugccguggaaaCAGCgccUCGGUUCg -3' miRNA: 3'- -CGACCgGGA-CGACAa------------GUUG---AGCCGAG- -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 12765 | 0.68 | 0.433578 |
Target: 5'- gGCcGcGCCCUGCUGggCAA--CGGCUa -3' miRNA: 3'- -CGaC-CGGGACGACaaGUUgaGCCGAg -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 10858 | 0.68 | 0.423773 |
Target: 5'- aGCaGGCCCUGCgugGcUUCGG-UCGGCg- -3' miRNA: 3'- -CGaCCGGGACGa--C-AAGUUgAGCCGag -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 12287 | 0.68 | 0.404566 |
Target: 5'- uGCUGGCCCaGCgugcCGGCUaCGGCg- -3' miRNA: 3'- -CGACCGGGaCGacaaGUUGA-GCCGag -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 14920 | 0.69 | 0.389603 |
Target: 5'- uGCUGGCCggccucaagugccgaCUGCgcgccCAACUCGGCg- -3' miRNA: 3'- -CGACCGG---------------GACGacaa-GUUGAGCCGag -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 25213 | 0.7 | 0.333484 |
Target: 5'- cGCUGGCCCgGCuUGUUgGug-CGGUUCc -3' miRNA: 3'- -CGACCGGGaCG-ACAAgUugaGCCGAG- -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 10048 | 0.73 | 0.207774 |
Target: 5'- uGCUGGCuuCCUGCUGggCGGCcggggccUCGGCg- -3' miRNA: 3'- -CGACCG--GGACGACaaGUUG-------AGCCGag -5' |
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26442 | 3' | -56.3 | NC_005357.1 | + | 41553 | 1.12 | 0.000292 |
Target: 5'- uGCUGGCCCUGCUGUUCAACUCGGCUCg -3' miRNA: 3'- -CGACCGGGACGACAAGUUGAGCCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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