Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26446 | 3' | -57.7 | NC_005357.1 | + | 27693 | 0.78 | 0.089846 |
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Target: 5'- uGCUGCGCGUacugccuGGgGCUACCgGGCGcAGGg -3' miRNA: 3'- -CGACGCGCA-------CCaCGAUGGgCUGC-UCC- -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 29769 | 0.67 | 0.478012 |
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Target: 5'- cCUGCGCaugaUGG-GCgacgACCCGACGccaaAGGa -3' miRNA: 3'- cGACGCGc---ACCaCGa---UGGGCUGC----UCC- -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 31304 | 0.66 | 0.561483 |
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Target: 5'- gGCgacCGCGUGGUG--GCCCGGCu-GGa -3' miRNA: 3'- -CGac-GCGCACCACgaUGGGCUGcuCC- -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 31845 | 0.66 | 0.550782 |
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Target: 5'- gGCUGCGCGccugGGUaCUACgCC--CGAGGc -3' miRNA: 3'- -CGACGCGCa---CCAcGAUG-GGcuGCUCC- -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 31892 | 0.67 | 0.458082 |
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Target: 5'- cGUUGCGCGUGGcgGCggaaagcgUGgCCGGCGGu- -3' miRNA: 3'- -CGACGCGCACCa-CG--------AUgGGCUGCUcc -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 34446 | 0.71 | 0.287065 |
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Target: 5'- --cGC-CGUGGUGCUgcuGCCUGACGAa- -3' miRNA: 3'- cgaCGcGCACCACGA---UGGGCUGCUcc -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 35337 | 0.66 | 0.529577 |
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Target: 5'- -gUGCGCGUGG-GC-GCgCCGugGguAGGc -3' miRNA: 3'- cgACGCGCACCaCGaUG-GGCugC--UCC- -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 39603 | 0.81 | 0.053836 |
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Target: 5'- cGCUGCGCGUGGccGCgagcaccugGCCCGACGAa- -3' miRNA: 3'- -CGACGCGCACCa-CGa--------UGGGCUGCUcc -5' |
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| 26446 | 3' | -57.7 | NC_005357.1 | + | 40211 | 1.12 | 0.000265 |
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Target: 5'- uGCUGCGCGUGGUGCUACCCGACGAGGg -3' miRNA: 3'- -CGACGCGCACCACGAUGGGCUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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