miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
26446 5' -56.6 NC_005357.1 + 40245 1.07 0.000662
Target:  5'- cAUCCCUAUCGGUAGCCUCACCUCGCAa -3'
miRNA:   3'- -UAGGGAUAGCCAUCGGAGUGGAGCGU- -5'
26446 5' -56.6 NC_005357.1 + 28656 0.68 0.417692
Target:  5'- aGUgCCagAUCGGUgccggcGGCCUCGCCagCGCGg -3'
miRNA:   3'- -UAgGGa-UAGCCA------UCGGAGUGGa-GCGU- -5'
26446 5' -56.6 NC_005357.1 + 18032 0.68 0.4235
Target:  5'- gGUUCUUGUCGGcgggcugcuugggGGCCUUACCggCGCGg -3'
miRNA:   3'- -UAGGGAUAGCCa------------UCGGAGUGGa-GCGU- -5'
26446 5' -56.6 NC_005357.1 + 10097 0.66 0.541976
Target:  5'- cAUCCUcgacaaaaagGUCGGUAguGCCUguCCUCGUAc -3'
miRNA:   3'- -UAGGGa---------UAGCCAU--CGGAguGGAGCGU- -5'
26446 5' -56.6 NC_005357.1 + 23208 0.66 0.552961
Target:  5'- -gCCUcGUCGGUgaauucGGCCUCgGCCUCGg- -3'
miRNA:   3'- uaGGGaUAGCCA------UCGGAG-UGGAGCgu -5'
26446 5' -56.6 NC_005357.1 + 8966 0.66 0.552961
Target:  5'- cAUCCgcacGUCGGUAacGCCUCGauuuCCUCGCc -3'
miRNA:   3'- -UAGGga--UAGCCAU--CGGAGU----GGAGCGu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.