Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2645 | 3' | -56.3 | NC_001491.2 | + | 28869 | 0.66 | 0.891216 |
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Target: 5'- --cGCCGgaGCUGgGCUgggcCGGGGUCg- -3' miRNA: 3'- ccaCGGUa-CGACgCGAa---GUCCCAGac -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 92967 | 0.66 | 0.884332 |
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Target: 5'- uGGUGCCucguUGUcauuggGcCGCUUCAGGG-CUc -3' miRNA: 3'- -CCACGGu---ACGa-----C-GCGAAGUCCCaGAc -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 69423 | 0.67 | 0.821556 |
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Target: 5'- --cGgCGUGCUGCGCcaCGGGuuGUCUGa -3' miRNA: 3'- ccaCgGUACGACGCGaaGUCC--CAGAC- -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 2738 | 0.68 | 0.803964 |
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Target: 5'- gGGUgGCCAccCUGCGCUUgGGGGcCg- -3' miRNA: 3'- -CCA-CGGUacGACGCGAAgUCCCaGac -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 5654 | 0.68 | 0.776413 |
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Target: 5'- cGUGCUA-GCUcCgGCUUCGGGGUCg- -3' miRNA: 3'- cCACGGUaCGAcG-CGAAGUCCCAGac -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 28840 | 0.69 | 0.737886 |
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Target: 5'- gGGUcGCCGgaGCUGgGCUgggcCGGGGUCg- -3' miRNA: 3'- -CCA-CGGUa-CGACgCGAa---GUCCCAGac -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 92101 | 0.69 | 0.718023 |
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Target: 5'- --cGCCGgcaucugGUUGCGCUggagCAGGGUCc- -3' miRNA: 3'- ccaCGGUa------CGACGCGAa---GUCCCAGac -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 25304 | 0.71 | 0.584854 |
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Target: 5'- aGGUGCCGUGCcGCGUgccggUCgAGGGggUGg -3' miRNA: 3'- -CCACGGUACGaCGCGa----AG-UCCCagAC- -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 7763 | 0.73 | 0.514416 |
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Target: 5'- cGGUGCCAUGC-GCGCUcccgagaCGGGGggUGg -3' miRNA: 3'- -CCACGGUACGaCGCGAa------GUCCCagAC- -5' |
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| 2645 | 3' | -56.3 | NC_001491.2 | + | 115352 | 1.1 | 0.001941 |
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Target: 5'- cGGUGCCAUGCUGCGCUUCAGGGUCUGu -3' miRNA: 3'- -CCACGGUACGACGCGAAGUCCCAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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