Results 101 - 108 of 108 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26450 | 3' | -56.4 | NC_005357.1 | + | 10743 | 0.68 | 0.480738 |
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Target: 5'- uUGCugCUCG-CCAagGGCGCGCucGa -3' miRNA: 3'- gAUGugGAGCuGGUg-CCGCGCGuuCc -5' |
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| 26450 | 3' | -56.4 | NC_005357.1 | + | 24626 | 0.68 | 0.480738 |
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Target: 5'- -gGCACgUCGAaccaCAUGGCGUGCcggcGGGc -3' miRNA: 3'- gaUGUGgAGCUg---GUGCCGCGCGu---UCC- -5' |
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| 26450 | 3' | -56.4 | NC_005357.1 | + | 28673 | 0.68 | 0.480738 |
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Target: 5'- -gGCgGCCUCG-CCagcGCGGCGCGCcacgucguucacAAGGu -3' miRNA: 3'- gaUG-UGGAGCuGG---UGCCGCGCG------------UUCC- -5' |
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| 26450 | 3' | -56.4 | NC_005357.1 | + | 9326 | 0.68 | 0.480738 |
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Target: 5'- -cGCACgUCGG-CGCGGCGgGCGGcGGc -3' miRNA: 3'- gaUGUGgAGCUgGUGCCGCgCGUU-CC- -5' |
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| 26450 | 3' | -56.4 | NC_005357.1 | + | 8688 | 0.68 | 0.490996 |
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Target: 5'- -cGCACCUCGGCguUGGUGUacugGCcGGGu -3' miRNA: 3'- gaUGUGGAGCUGguGCCGCG----CGuUCC- -5' |
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| 26450 | 3' | -56.4 | NC_005357.1 | + | 40210 | 0.68 | 0.490996 |
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Target: 5'- uUAUACCgCGA-CAUGGaGCGCGAGGc -3' miRNA: 3'- gAUGUGGaGCUgGUGCCgCGCGUUCC- -5' |
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| 26450 | 3' | -56.4 | NC_005357.1 | + | 19539 | 0.68 | 0.501356 |
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Target: 5'- gUugGCCUCGAUgACGGUcauGuCGuCGAGGa -3' miRNA: 3'- gAugUGGAGCUGgUGCCG---C-GC-GUUCC- -5' |
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| 26450 | 3' | -56.4 | NC_005357.1 | + | 14810 | 0.66 | 0.609085 |
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Target: 5'- cCUGCACCUCG---GCGGCGCuGCc--- -3' miRNA: 3'- -GAUGUGGAGCuggUGCCGCG-CGuucc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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