Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26452 | 5' | -55.8 | NC_005357.1 | + | 30852 | 0.79 | 0.098335 |
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Target: 5'- cGGCgGcCCUGGGCGGCAUgGCuucgAUGACc -3' miRNA: 3'- -CCGaCuGGACCCGCCGUAgCG----UAUUG- -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 32225 | 0.67 | 0.552961 |
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Target: 5'- cGCcGACCaucGGGcCGGCGUgCGCuUGACg -3' miRNA: 3'- cCGaCUGGa--CCC-GCCGUA-GCGuAUUG- -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 33746 | 0.71 | 0.320321 |
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Target: 5'- cGGCgcacACCUGGGCGGCGcaGCAgccGCu -3' miRNA: 3'- -CCGac--UGGACCCGCCGUagCGUau-UG- -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 34367 | 0.72 | 0.267761 |
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Target: 5'- aGGCagcGACCUGGGCGcGCGUCauggacggcgGCAagGACg -3' miRNA: 3'- -CCGa--CUGGACCCGC-CGUAG----------CGUa-UUG- -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 35182 | 0.73 | 0.240979 |
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Target: 5'- ---aGACCUGGGCGGCcgacAUCGCcgAGg -3' miRNA: 3'- ccgaCUGGACCCGCCG----UAGCGuaUUg -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 35984 | 0.67 | 0.498841 |
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Target: 5'- cGGCgacGCCUucgcGGGCGGUgguGUCGCG-AGCg -3' miRNA: 3'- -CCGac-UGGA----CCCGCCG---UAGCGUaUUG- -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 36902 | 0.71 | 0.304553 |
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Target: 5'- gGGCgGaACCUGGGCGGCcg-GCGUGccuACg -3' miRNA: 3'- -CCGaC-UGGACCCGCCGuagCGUAU---UG- -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 37725 | 0.68 | 0.447209 |
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Target: 5'- gGGCU-ACgaGGGCGGCGUCGg----- -3' miRNA: 3'- -CCGAcUGgaCCCGCCGUAGCguauug -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 37833 | 1.11 | 0.000393 |
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Target: 5'- uGGCUGACCUGGGCGGCAUCGCAUAACu -3' miRNA: 3'- -CCGACUGGACCCGCCGUAGCGUAUUG- -5' |
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| 26452 | 5' | -55.8 | NC_005357.1 | + | 41620 | 0.67 | 0.552961 |
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Target: 5'- cGGCaaGGgCUGGGCGcggcGCGUCGCcgGAa -3' miRNA: 3'- -CCGa-CUgGACCCGC----CGUAGCGuaUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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