Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26453 | 5' | -50.6 | NC_005357.1 | + | 3455 | 0.66 | 0.913886 |
Target: 5'- aGGCAACCgAUGGaaccCAUCGaggaagcgacaaaguGCUACGa -3' miRNA: 3'- cCCGUUGGaUACCa---GUAGC---------------UGAUGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 5866 | 0.66 | 0.911239 |
Target: 5'- aGGGCGGCUUcuaccccaaGGUCAUCGAgcACGc -3' miRNA: 3'- -CCCGUUGGAua-------CCAGUAGCUgaUGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 35302 | 0.66 | 0.904415 |
Target: 5'- gGGGCGACgCUG-GGcuugcCAUCGgACUGCGu -3' miRNA: 3'- -CCCGUUG-GAUaCCa----GUAGC-UGAUGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 4894 | 0.66 | 0.902311 |
Target: 5'- cGGCGGCCUcguuuucggccacgGUGGaaaCGUCGGCaGCGa -3' miRNA: 3'- cCCGUUGGA--------------UACCa--GUAGCUGaUGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 32432 | 0.66 | 0.889896 |
Target: 5'- uGGGCGGCCgg-GGcC-UCGGCgGCGa -3' miRNA: 3'- -CCCGUUGGauaCCaGuAGCUGaUGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 8125 | 0.67 | 0.874248 |
Target: 5'- -cGCGGCCggcguUGGUUGUgGGCgUGCGGg -3' miRNA: 3'- ccCGUUGGau---ACCAGUAgCUG-AUGCC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 23429 | 0.67 | 0.874248 |
Target: 5'- cGGGCAcGCCaccGUGuUCAUCGGCgGCGa -3' miRNA: 3'- -CCCGU-UGGa--UACcAGUAGCUGaUGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 20330 | 0.67 | 0.857521 |
Target: 5'- cGGGCuGGCCUucaGUGGUCGUugCGGCUucCGu -3' miRNA: 3'- -CCCG-UUGGA---UACCAGUA--GCUGAu-GCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 39970 | 0.67 | 0.857521 |
Target: 5'- uGGGCGugCc--GGUCAguUCGAUgaaGCGGu -3' miRNA: 3'- -CCCGUugGauaCCAGU--AGCUGa--UGCC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 37759 | 0.68 | 0.830549 |
Target: 5'- gGGGCGgcuACCUAcGG-CAUCGACcuugACGc -3' miRNA: 3'- -CCCGU---UGGAUaCCaGUAGCUGa---UGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 34692 | 0.68 | 0.830549 |
Target: 5'- uGGGCAugUcccacucgcugaUAUGGUCGaggcgCGGCUGCa- -3' miRNA: 3'- -CCCGUugG------------AUACCAGUa----GCUGAUGcc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 25632 | 0.68 | 0.821096 |
Target: 5'- cGGCGuacaacuccgacGCCgaca-UCAUCGGCUACGGg -3' miRNA: 3'- cCCGU------------UGGauaccAGUAGCUGAUGCC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 4531 | 0.68 | 0.808487 |
Target: 5'- uGGCGugCUGggcGGUCGugccgccgcggaacUgGACUGCGGu -3' miRNA: 3'- cCCGUugGAUa--CCAGU--------------AgCUGAUGCC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 22768 | 0.69 | 0.760285 |
Target: 5'- aGGGUgagGGCCgauUGGUCAaCGccCUGCGGg -3' miRNA: 3'- -CCCG---UUGGau-ACCAGUaGCu-GAUGCC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 28141 | 0.7 | 0.694392 |
Target: 5'- cGGGCGgcagcagcuuGCCgGUGGUCAgaUCGACcACGc -3' miRNA: 3'- -CCCGU----------UGGaUACCAGU--AGCUGaUGCc -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 32945 | 0.71 | 0.648994 |
Target: 5'- aGGGCGACCac-GGcugCGUCGAUUACuGGc -3' miRNA: 3'- -CCCGUUGGauaCCa--GUAGCUGAUG-CC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 9312 | 0.74 | 0.492247 |
Target: 5'- cGGCGACCUGgcGGcgcaCGUCGGCgcgGCGGg -3' miRNA: 3'- cCCGUUGGAUa-CCa---GUAGCUGa--UGCC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 38389 | 0.74 | 0.471091 |
Target: 5'- uGGGCGGCgCUGaauucgucgcGGUCGUCGGCUuCGGu -3' miRNA: 3'- -CCCGUUG-GAUa---------CCAGUAGCUGAuGCC- -5' |
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26453 | 5' | -50.6 | NC_005357.1 | + | 4909 | 1.13 | 0.001358 |
Target: 5'- cGGGCAACCUAUGGUCAUCGACUACGGc -3' miRNA: 3'- -CCCGUUGGAUACCAGUAGCUGAUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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