miRNA: 3'- aCaGCGGUUGCUGUAgCUGuCGCacc -5'
miRNA: 3'- -ACAGCGGU--UGCUgUaGCUGUCGCaCC- -5'
miRNA: 3'- acAGCGGUUGC--------UGuaGCU----GUCGCACC- -5'
miRNA: 3'- -ACAGCGGUUGCUGUAGCUGUCGCACC- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26456 | 5' | -53.1 | NC_005357.1 | + | 36013 | 0.71 | 0.498841 |
|
Target: 5'- aGgCGCCAACGACAUgGGCcGCGa-- -3' miRNA: 3'- aCaGCGGUUGCUGUAgCUGuCGCacc -5' |
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| 26456 | 5' | -53.1 | NC_005357.1 | + | 27146 | 0.71 | 0.47785 |
|
Target: 5'- cUGUCGCCGaaAUGGgG-CGACAGCG-GGc -3' miRNA: 3'- -ACAGCGGU--UGCUgUaGCUGUCGCaCC- -5' |
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| 26456 | 5' | -53.1 | NC_005357.1 | + | 8949 | 0.72 | 0.457303 |
|
Target: 5'- -uUCGCCAGCGguggccguGCcgCGAuagcCAGCGUGGc -3' miRNA: 3'- acAGCGGUUGC--------UGuaGCU----GUCGCACC- -5' |
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| 26456 | 5' | -53.1 | NC_005357.1 | + | 37086 | 1.1 | 0.001114 |
|
Target: 5'- cUGUCGCCAACGACAUCGACAGCGUGGu -3' miRNA: 3'- -ACAGCGGUUGCUGUAGCUGUCGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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