miRNA: 3'- aCAGCGGuUGCUGUAGCUGUCGcacc -5'
miRNA: 3'- aCAGCGGU------UGCUGUagCUGUCGCACC- -5'
miRNA: 3'- aCaGCGGUUGCUGUAGCU-GUCGCACc -5'
miRNA: 3'- -ACAGCGGUUGCUGUAGCUGUCGCACC- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26456 | 5' | -53.1 | NC_005357.1 | + | 6297 | 0.8 | 0.138834 |
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Target: 5'- gGUCGCCcACGACAUUGACGGCa--- -3' miRNA: 3'- aCAGCGGuUGCUGUAGCUGUCGcacc -5' |
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| 26456 | 5' | -53.1 | NC_005357.1 | + | 36434 | 0.8 | 0.134944 |
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Target: 5'- cGUCGCCAuuuugaGCGACGcaGACGGUGUGGa -3' miRNA: 3'- aCAGCGGU------UGCUGUagCUGUCGCACC- -5' |
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| 26456 | 5' | -53.1 | NC_005357.1 | + | 19049 | 0.85 | 0.063302 |
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Target: 5'- gGcCGCCGGCGGCGUCGAuCAGCGUGu -3' miRNA: 3'- aCaGCGGUUGCUGUAGCU-GUCGCACc -5' |
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| 26456 | 5' | -53.1 | NC_005357.1 | + | 37086 | 1.1 | 0.001114 |
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Target: 5'- cUGUCGCCAACGACAUCGACAGCGUGGu -3' miRNA: 3'- -ACAGCGGUUGCUGUAGCUGUCGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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