Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26457 | 3' | -54.6 | NC_005357.1 | + | 24802 | 0.66 | 0.703967 |
Target: 5'- cGCGUUGAaguCGCCGUacagcaucuGGCUGCgCAAUGGc -3' miRNA: 3'- -UGUAGCU---GCGGCA---------CCGAUG-GUUGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 32656 | 0.66 | 0.703967 |
Target: 5'- --uUCGGCGCCGcgaaGCUGCCcGACGuGa -3' miRNA: 3'- uguAGCUGCGGCac--CGAUGG-UUGCuC- -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 24628 | 0.66 | 0.703967 |
Target: 5'- cACGUCGAa-CCacaUGGCgUGCCGGCGGGc -3' miRNA: 3'- -UGUAGCUgcGGc--ACCG-AUGGUUGCUC- -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 24788 | 0.66 | 0.692924 |
Target: 5'- -gAUCGugGCCGUa-CUGCCGcACGAu -3' miRNA: 3'- ugUAGCugCGGCAccGAUGGU-UGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 13733 | 0.66 | 0.690707 |
Target: 5'- aGCAUCGACcuugugaacgaCGUGGCgcgccgcGCUGGCGAGg -3' miRNA: 3'- -UGUAGCUGcg---------GCACCGa------UGGUUGCUC- -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 13659 | 0.66 | 0.681819 |
Target: 5'- aGCG-CGACGCCGUGcGC-GCCGA-GAa -3' miRNA: 3'- -UGUaGCUGCGGCAC-CGaUGGUUgCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 36033 | 0.66 | 0.681819 |
Target: 5'- gACAUCaccgagGGCGCCGcGGCgGCCGACc-- -3' miRNA: 3'- -UGUAG------CUGCGGCaCCGaUGGUUGcuc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 18411 | 0.66 | 0.681819 |
Target: 5'- cACGUCGGCGaagGUGGCguuguCCGcCGAGu -3' miRNA: 3'- -UGUAGCUGCgg-CACCGau---GGUuGCUC- -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 33654 | 0.66 | 0.670665 |
Target: 5'- cCAUCGuGCGCCGcGuGCUGCCcgaAGCGGc -3' miRNA: 3'- uGUAGC-UGCGGCaC-CGAUGG---UUGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 8455 | 0.66 | 0.659473 |
Target: 5'- gAUGUCGGCGUCGaGGUgGCCuGCGAu -3' miRNA: 3'- -UGUAGCUGCGGCaCCGaUGGuUGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 5346 | 0.67 | 0.648254 |
Target: 5'- -uGUCGAUGUCGuUGGCgacaGCCAACa-- -3' miRNA: 3'- ugUAGCUGCGGC-ACCGa---UGGUUGcuc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 36877 | 0.67 | 0.63702 |
Target: 5'- cCGUCGAUaCCGUGGCgggcgACCuggGCGGa -3' miRNA: 3'- uGUAGCUGcGGCACCGa----UGGu--UGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 35982 | 0.67 | 0.63702 |
Target: 5'- gACggCGACGCCuucgcgggcgGUGGUgucGCgAGCGAGg -3' miRNA: 3'- -UGuaGCUGCGG----------CACCGa--UGgUUGCUC- -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 27581 | 0.67 | 0.625782 |
Target: 5'- aGCGUgCGGCGC--UGGCcGCCAACGGc -3' miRNA: 3'- -UGUA-GCUGCGgcACCGaUGGUUGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 25658 | 0.67 | 0.614549 |
Target: 5'- cCAUCGccuucacguCGCCgGUGGCUacGCCcACGAGc -3' miRNA: 3'- uGUAGCu--------GCGG-CACCGA--UGGuUGCUC- -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 18670 | 0.67 | 0.603332 |
Target: 5'- cGCAUCGGC-CCgGUGGC-GCCGGCa-- -3' miRNA: 3'- -UGUAGCUGcGG-CACCGaUGGUUGcuc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 5638 | 0.67 | 0.592141 |
Target: 5'- gGCGagGugGCCGUacacGGCggaaACCAGCGGu -3' miRNA: 3'- -UGUagCugCGGCA----CCGa---UGGUUGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 29925 | 0.67 | 0.592141 |
Target: 5'- cGCAUCagUGCCGUGGCUuGCCAGucAGg -3' miRNA: 3'- -UGUAGcuGCGGCACCGA-UGGUUgcUC- -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 37097 | 0.67 | 0.592141 |
Target: 5'- gACAUCGACaG-CGUGGUauccgugGCCGGCGAc -3' miRNA: 3'- -UGUAGCUG-CgGCACCGa------UGGUUGCUc -5' |
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26457 | 3' | -54.6 | NC_005357.1 | + | 39254 | 0.67 | 0.592141 |
Target: 5'- cGCAgcgGACGCCG-GGCuUGCCAugcuGCGAu -3' miRNA: 3'- -UGUag-CUGCGGCaCCG-AUGGU----UGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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