Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26460 | 3' | -59.9 | NC_005357.1 | + | 7148 | 0.67 | 0.346306 |
Target: 5'- cGGCUGCGgccuggaugacCAGGGCcg-GGCGCuuGUCGg -3' miRNA: 3'- -CCGACGCa----------GUCCCGcagUCGCG--CAGC- -5' |
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26460 | 3' | -59.9 | NC_005357.1 | + | 4859 | 0.67 | 0.36292 |
Target: 5'- aGGUUGCccgGUCAGGuCGUugccCAGCGCGgCGa -3' miRNA: 3'- -CCGACG---CAGUCCcGCA----GUCGCGCaGC- -5' |
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26460 | 3' | -59.9 | NC_005357.1 | + | 1631 | 0.66 | 0.4347 |
Target: 5'- gGGC-GUGUCguacccaucGGGGUagGUCuGCGCGUCc -3' miRNA: 3'- -CCGaCGCAG---------UCCCG--CAGuCGCGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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