miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
26460 5' -51.3 NC_005357.1 + 7523 0.66 0.824077
Target:  5'- uUUCGGCGUCc-GAGUGUUCUUccGCAc -3'
miRNA:   3'- -AAGUUGCAGuaCUCGCGGGAAuaCGU- -5'
26460 5' -51.3 NC_005357.1 + 33177 0.66 0.824077
Target:  5'- ---uGCGgaaGUGGGCGCCCUcg-GCAc -3'
miRNA:   3'- aaguUGCag-UACUCGCGGGAauaCGU- -5'
26460 5' -51.3 NC_005357.1 + 28721 0.66 0.783184
Target:  5'- gUCGGCG-CAcgcgaggaUGAGCGCCUUg--GCAu -3'
miRNA:   3'- aAGUUGCaGU--------ACUCGCGGGAauaCGU- -5'
26460 5' -51.3 NC_005357.1 + 8001 0.68 0.716737
Target:  5'- -aCGGCGUCG-GAcGUGCCCUUG-GCGc -3'
miRNA:   3'- aaGUUGCAGUaCU-CGCGGGAAUaCGU- -5'
26460 5' -51.3 NC_005357.1 + 13166 0.68 0.669159
Target:  5'- gUCGGCcaguGUCGgccgcgcUGGGCGCCCUUGgccgGCGu -3'
miRNA:   3'- aAGUUG----CAGU-------ACUCGCGGGAAUa---CGU- -5'
26460 5' -51.3 NC_005357.1 + 42019 0.69 0.663293
Target:  5'- -cCGACGaUCAccgccgugcugcuggUGGGCGCCCUgg-GCAu -3'
miRNA:   3'- aaGUUGC-AGU---------------ACUCGCGGGAauaCGU- -5'
26460 5' -51.3 NC_005357.1 + 39637 0.69 0.599731
Target:  5'- aUCGACGaaGUGGGCGCaCCUgc-GCAa -3'
miRNA:   3'- aAGUUGCagUACUCGCG-GGAauaCGU- -5'
26460 5' -51.3 NC_005357.1 + 36137 0.71 0.530225
Target:  5'- gUCAAUGUCGUGGGCGaCCUgUUcgGCc -3'
miRNA:   3'- aAGUUGCAGUACUCGC-GGG-AAuaCGu -5'
26460 5' -51.3 NC_005357.1 + 13765 0.71 0.485539
Target:  5'- -cCAGCGccgaCAUGAGCGCaauCUUGUGCGc -3'
miRNA:   3'- aaGUUGCa---GUACUCGCGg--GAAUACGU- -5'
26460 5' -51.3 NC_005357.1 + 10407 0.76 0.287633
Target:  5'- gUUCAAUGUCGUGGGCGUCCagcGUGUc -3'
miRNA:   3'- -AAGUUGCAGUACUCGCGGGaa-UACGu -5'
26460 5' -51.3 NC_005357.1 + 35285 1.07 0.002013
Target:  5'- cUUCAACGUCAUGAGCGCCCUUAUGCAa -3'
miRNA:   3'- -AAGUUGCAGUACUCGCGGGAAUACGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.