Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26461 | 5' | -59.3 | NC_005357.1 | + | 8269 | 0.69 | 0.281367 |
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Target: 5'- cCGGGCAUU-CGGCuUCCaGGGCCAcGCu -3' miRNA: 3'- -GCUCGUGAuGCCG-GGGcUCCGGUuCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 7386 | 0.68 | 0.341408 |
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Target: 5'- gCGGGCACcGCGGCgaccuuccaccagCuuGAaGCCGGGCa -3' miRNA: 3'- -GCUCGUGaUGCCG-------------GggCUcCGGUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 7276 | 0.67 | 0.376036 |
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Target: 5'- ----uGCUGCuuGGCCUCGGGGCCGuAGUg -3' miRNA: 3'- gcucgUGAUG--CCGGGGCUCCGGU-UCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 7138 | 0.71 | 0.211572 |
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Target: 5'- gCGaAGUcgucgGCUGCGGCCUgGAugaccagGGCCGGGCg -3' miRNA: 3'- -GC-UCG-----UGAUGCCGGGgCU-------CCGGUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 4883 | 0.67 | 0.393766 |
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Target: 5'- -cAGCGCggcgacgGCGGCCUCGuuuucGGCCAcGGUg -3' miRNA: 3'- gcUCGUGa------UGCCGGGGCu----CCGGU-UCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 1960 | 0.8 | 0.045576 |
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Target: 5'- uCGAGCGC-GCGGCCCUGGGGgaAGGCa -3' miRNA: 3'- -GCUCGUGaUGCCGGGGCUCCggUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 1462 | 0.67 | 0.350468 |
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Target: 5'- -cGGCGCggucgGCGGCCUCcuggcAGGCCGGGg -3' miRNA: 3'- gcUCGUGa----UGCCGGGGc----UCCGGUUCg -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 1314 | 0.68 | 0.318342 |
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Target: 5'- aGGGCG--ACGGCCaCC-AGGCCGacaAGCg -3' miRNA: 3'- gCUCGUgaUGCCGG-GGcUCCGGU---UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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