Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26464 | 5' | -52.6 | NC_005357.1 | + | 18050 | 0.72 | 0.407639 |
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Target: 5'- aGCGAguUGUGGUGGCgaaUGCGCGAaGCc -3' miRNA: 3'- -CGCUguACGUCACCG---ACGUGUUaCGu -5' |
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| 26464 | 5' | -52.6 | NC_005357.1 | + | 37254 | 0.73 | 0.369654 |
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Target: 5'- aCGACGUGCAGgcGGUgGCagGCAAUGCGg -3' miRNA: 3'- cGCUGUACGUCa-CCGaCG--UGUUACGU- -5' |
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| 26464 | 5' | -52.6 | NC_005357.1 | + | 14631 | 0.73 | 0.334164 |
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Target: 5'- -gGACGUGCuGcGGCUGCGCGGcGCGc -3' miRNA: 3'- cgCUGUACGuCaCCGACGUGUUaCGU- -5' |
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| 26464 | 5' | -52.6 | NC_005357.1 | + | 14958 | 0.74 | 0.293402 |
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Target: 5'- cGCGugGUGC-GUGGCgGCGCGcugGCAu -3' miRNA: 3'- -CGCugUACGuCACCGaCGUGUua-CGU- -5' |
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| 26464 | 5' | -52.6 | NC_005357.1 | + | 35961 | 0.77 | 0.183718 |
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Target: 5'- cGCGGCGUGCAGUucCUGCGCGAcgGCGa -3' miRNA: 3'- -CGCUGUACGUCAccGACGUGUUa-CGU- -5' |
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| 26464 | 5' | -52.6 | NC_005357.1 | + | 33041 | 1.11 | 0.000757 |
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Target: 5'- cGCGACAUGCAGUGGCUGCACAAUGCAc -3' miRNA: 3'- -CGCUGUACGUCACCGACGUGUUACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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