Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26466 | 5' | -57.6 | NC_005357.1 | + | 36143 | 0.66 | 0.450706 |
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Target: 5'- gUCGuGGGCGAccuguucGGCcuGCCCAGCGc--- -3' miRNA: 3'- aAGCuCCCGUU-------CCG--CGGGUCGUaacu -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 7675 | 0.66 | 0.441669 |
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Target: 5'- -cCGGGGGCcAGGuCGUCgGGC-UUGAc -3' miRNA: 3'- aaGCUCCCGuUCC-GCGGgUCGuAACU- -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 16468 | 0.66 | 0.437686 |
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Target: 5'- aUCGGGGGCGugacggacaggucGGGCGCgauauugucguccaCCAGCGa--- -3' miRNA: 3'- aAGCUCCCGU-------------UCCGCG--------------GGUCGUaacu -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 23572 | 0.67 | 0.412309 |
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Target: 5'- -aCGuGGGCAuGGGcCGCCCGGCc---- -3' miRNA: 3'- aaGCuCCCGU-UCC-GCGGGUCGuaacu -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 29246 | 0.68 | 0.340227 |
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Target: 5'- -aCGccGGCcaAGGGCGCCCAGCGc--- -3' miRNA: 3'- aaGCucCCG--UUCCGCGGGUCGUaacu -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 10305 | 0.7 | 0.25676 |
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Target: 5'- aUUCGGGGGCGucgAGGUcggcagcgGCCagGGCGUUGAg -3' miRNA: 3'- -AAGCUCCCGU---UCCG--------CGGg-UCGUAACU- -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 16931 | 0.71 | 0.230846 |
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Target: 5'- uUUCGuAGGGCAcGGCGCcguCCAGCAg--- -3' miRNA: 3'- -AAGC-UCCCGUuCCGCG---GGUCGUaacu -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 27561 | 0.72 | 0.185628 |
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Target: 5'- ----cGGGCAacuuaaAGGCGCCCAGCGUg-- -3' miRNA: 3'- aagcuCCCGU------UCCGCGGGUCGUAacu -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 32422 | 0.72 | 0.175614 |
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Target: 5'- -gCGGGGGCcGGGCGCgCGGCAc--- -3' miRNA: 3'- aaGCUCCCGuUCCGCGgGUCGUaacu -5' |
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| 26466 | 5' | -57.6 | NC_005357.1 | + | 32735 | 1.06 | 0.000487 |
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Target: 5'- cUUCGAGGGCAAGGCGCCCAGCAUUGAa -3' miRNA: 3'- -AAGCUCCCGUUCCGCGGGUCGUAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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