miRNA: 3'- -GGACCCU---UUUCu----GGGCguU-ACUGGcu -5'
miRNA: 3'- ggaCCCUUUUCuGGgCGUUACUGgcu -5'
miRNA: 3'- -GGACCCuuuUCUGG-GCGuuACUGGCu -5'
miRNA: 3'- gGACCCUUU--------------UCUGGG--CGuUACuGGCU- -5'
miRNA: 3'- ggaCCCUUU--UCUG-------GGCGuUACUGGCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2647 | 3' | -51.9 | NC_001491.2 | + | 68972 | 0.66 | 0.987776 |
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Target: 5'- cCCUGGGGcccAAGGcgauuCCCGguGuUGACCa- -3' miRNA: 3'- -GGACCCU---UUUCu----GGGCguU-ACUGGcu -5' |
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| 2647 | 3' | -51.9 | NC_001491.2 | + | 116 | 0.66 | 0.989232 |
|
Target: 5'- --gGGGAGGAGuCCgGUAGUGACg-- -3' miRNA: 3'- ggaCCCUUUUCuGGgCGUUACUGgcu -5' |
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| 2647 | 3' | -51.9 | NC_001491.2 | + | 61907 | 0.66 | 0.989232 |
|
Target: 5'- uCCUGGG---GGACCaUGC--UGACCGu -3' miRNA: 3'- -GGACCCuuuUCUGG-GCGuuACUGGCu -5' |
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| 2647 | 3' | -51.9 | NC_001491.2 | + | 146537 | 0.66 | 0.990168 |
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Target: 5'- gCUGGGAGAguugcacucuucgcGGGCCCuuGCcAUG-CCGGa -3' miRNA: 3'- gGACCCUUU--------------UCUGGG--CGuUACuGGCU- -5' |
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| 2647 | 3' | -51.9 | NC_001491.2 | + | 33361 | 0.66 | 0.990424 |
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Target: 5'- --gGGGAGGccGGACucacucgCCGCcGUGGCCGGc -3' miRNA: 3'- ggaCCCUUU--UCUG-------GGCGuUACUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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