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miRNA: 3'- ggCUGCGGCUC-Ca--GCUCGUCG-----UGGAg -5'
miRNA: 3'- gGCUGCGGCUCCA---------------GCU------CGUCGUGGag -5'
miRNA: 3'- gGCuGCggCUCCAGCUcGUCgUGGAG- -5'
miRNA: 3'- gGCUgCGGCUcCAGCUCGuCGUGGag -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 26473 | 3' | -59.2 | NC_005357.1 | + | 34512 | 0.66 | 0.435404 |
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Target: 5'- -gGACGCCGAGcGcaaCGAGCAGCuggcgAUCUa -3' miRNA: 3'- ggCUGCGGCUC-Ca--GCUCGUCG-----UGGAg -5' |
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| 26473 | 3' | -59.2 | NC_005357.1 | + | 13902 | 0.66 | 0.429713 |
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Target: 5'- aUGGCGUCGGGGUuguuccaguucagggCGAugucgcGCAGCGCCg- -3' miRNA: 3'- gGCUGCGGCUCCA---------------GCU------CGUCGUGGag -5' |
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| 26473 | 3' | -59.2 | NC_005357.1 | + | 19366 | 0.66 | 0.425943 |
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Target: 5'- uUGcCGuuGAGGUCGGcCAGgGCCUUg -3' miRNA: 3'- gGCuGCggCUCCAGCUcGUCgUGGAG- -5' |
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| 26473 | 3' | -59.2 | NC_005357.1 | + | 31674 | 0.66 | 0.464491 |
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Target: 5'- gCGG-GCCGAaGUCGAGC-GCGCCc- -3' miRNA: 3'- gGCUgCGGCUcCAGCUCGuCGUGGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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