miRNA: 3'- -GgCUGCGGCUCCAGCUCGuCGUGGaG- -5'
miRNA: 3'- gGCUGCGGCUCCAGCUC-GuCGUGGag -5'
miRNA: 3'- -GGCU------GCGGCUCCaGCUCGUCGuGGAG- -5'
miRNA: 3'- -GGCUGCGGCUCCAGCUCGUCGUGGAG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26473 | 3' | -59.2 | NC_005357.1 | + | 15974 | 0.8 | 0.054079 |
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Target: 5'- aCgGAUGCCGAGGUCGAGC-GCAUC-Cg -3' miRNA: 3'- -GgCUGCGGCUCCAGCUCGuCGUGGaG- -5' |
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| 26473 | 3' | -59.2 | NC_005357.1 | + | 35077 | 0.81 | 0.039394 |
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Target: 5'- aCGACGCCGAGGUCGAGgCcGCGCUc- -3' miRNA: 3'- gGCUGCGGCUCCAGCUC-GuCGUGGag -5' |
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| 26473 | 3' | -59.2 | NC_005357.1 | + | 37822 | 0.82 | 0.034085 |
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Target: 5'- gCCGAcaucauCGCCGAGGcCGAGCAGUuCCUCg -3' miRNA: 3'- -GGCU------GCGGCUCCaGCUCGUCGuGGAG- -5' |
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| 26473 | 3' | -59.2 | NC_005357.1 | + | 29896 | 1.12 | 0.000205 |
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Target: 5'- aCCGACGCCGAGGUCGAGCAGCACCUCg -3' miRNA: 3'- -GGCUGCGGCUCCAGCUCGUCGUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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