Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26476 | 5' | -55.8 | NC_005357.1 | + | 4172 | 0.74 | 0.222648 |
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Target: 5'- cGCCGugGUCGAGGUGUAGccaGUGGGc -3' miRNA: 3'- cCGGCugUAGCUCCGCGUCaa-CGUCU- -5' |
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| 26476 | 5' | -55.8 | NC_005357.1 | + | 35191 | 0.75 | 0.18931 |
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Target: 5'- cGGCCGACAUCGccgaggguGaGCGCGGc-GCAGAu -3' miRNA: 3'- -CCGGCUGUAGCu-------C-CGCGUCaaCGUCU- -5' |
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| 26476 | 5' | -55.8 | NC_005357.1 | + | 13311 | 0.75 | 0.1842 |
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Target: 5'- gGGCCGGCGUUGuaggcGGCGUAGgcuuucgGCAGGu -3' miRNA: 3'- -CCGGCUGUAGCu----CCGCGUCaa-----CGUCU- -5' |
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| 26476 | 5' | -55.8 | NC_005357.1 | + | 7817 | 0.77 | 0.139481 |
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Target: 5'- cGCUGAUaugGUCGAGGCGCGGcUGCAcGAu -3' miRNA: 3'- cCGGCUG---UAGCUCCGCGUCaACGU-CU- -5' |
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| 26476 | 5' | -55.8 | NC_005357.1 | + | 23560 | 0.78 | 0.111117 |
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Target: 5'- cGGCCGACAUCGccggcAGGUGCGG--GCGGGg -3' miRNA: 3'- -CCGGCUGUAGC-----UCCGCGUCaaCGUCU- -5' |
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| 26476 | 5' | -55.8 | NC_005357.1 | + | 29279 | 1.11 | 0.000452 |
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Target: 5'- uGGCCGACAUCGAGGCGCAGUUGCAGAa -3' miRNA: 3'- -CCGGCUGUAGCUCCGCGUCAACGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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