Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 26485 | 3' | -54.6 | NC_005357.1 | + | 42022 | 0.66 | 0.640211 |
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Target: 5'- cGCgACGUGCugcucaacagucGCGcGCUGGCCgcgcuCAAUc -3' miRNA: 3'- -CGgUGCACG------------CGCuUGACCGGau---GUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 25194 | 0.66 | 0.651522 |
|
Target: 5'- uGUCAUGagcacGCGCGAccGCUGGCC--CGGCu -3' miRNA: 3'- -CGGUGCa----CGCGCU--UGACCGGauGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 25616 | 0.66 | 0.662815 |
|
Target: 5'- aCCACGgcgGCGCGGuc--GCCaGCAGCg -3' miRNA: 3'- cGGUGCa--CGCGCUugacCGGaUGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 28874 | 0.66 | 0.662815 |
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Target: 5'- cGCCACGUccucgcugcugGCGUaguuGCUGGCCgucgcCGACc -3' miRNA: 3'- -CGGUGCA-----------CGCGcu--UGACCGGau---GUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 24140 | 0.66 | 0.685297 |
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Target: 5'- aGCCGgcUGCGCGc-CUGGCC--CAGCg -3' miRNA: 3'- -CGGUgcACGCGCuuGACCGGauGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 38551 | 0.66 | 0.685297 |
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Target: 5'- gGCgACG-GCaGCGc---GGCCUACAGCg -3' miRNA: 3'- -CGgUGCaCG-CGCuugaCCGGAUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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