Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26485 | 3' | -54.6 | NC_005357.1 | + | 5198 | 0.75 | 0.213922 |
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Target: 5'- -gCugGUGCugGUGGuGCUGGCCUGCAACc -3' miRNA: 3'- cgGugCACG--CGCU-UGACCGGAUGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 13864 | 0.75 | 0.208167 |
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Target: 5'- gGCCgACGUGCGCGccgcgaUGGCCgGCGGCc -3' miRNA: 3'- -CGG-UGCACGCGCuug---ACCGGaUGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 25627 | 0.75 | 0.202547 |
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Target: 5'- cGCCugG-GCGCGAccaACUGGCaacugGCGACc -3' miRNA: 3'- -CGGugCaCGCGCU---UGACCGga---UGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 37111 | 0.76 | 0.197059 |
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Target: 5'- cGCCACGUGCuCGc---GGCCUACGGCc -3' miRNA: 3'- -CGGUGCACGcGCuugaCCGGAUGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 17748 | 0.79 | 0.115131 |
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Target: 5'- aGCCugG-GCGUGGACgUGGCCcGCGGCg -3' miRNA: 3'- -CGGugCaCGCGCUUG-ACCGGaUGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 26379 | 1.11 | 0.000544 |
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Target: 5'- gGCCACGUGCGCGAACUGGCCUACAACu -3' miRNA: 3'- -CGGUGCACGCGCUUGACCGGAUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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