Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26485 | 3' | -54.6 | NC_005357.1 | + | 39583 | 0.73 | 0.272033 |
Target: 5'- cGCUACG-GCuugGCGGGCUGGCCUuuccuCAAUa -3' miRNA: 3'- -CGGUGCaCG---CGCUUGACCGGAu----GUUG- -5' |
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26485 | 3' | -54.6 | NC_005357.1 | + | 39600 | 0.66 | 0.640211 |
Target: 5'- cGCCgcugcGCGUGgcCGCGAGCaccUGGCCcgACGAa -3' miRNA: 3'- -CGG-----UGCAC--GCGCUUG---ACCGGa-UGUUg -5' |
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26485 | 3' | -54.6 | NC_005357.1 | + | 41287 | 0.73 | 0.271323 |
Target: 5'- cGCCGcCGUaGCGCGAGCUaacgguuacaccgGGCCgaugcGCGACa -3' miRNA: 3'- -CGGU-GCA-CGCGCUUGA-------------CCGGa----UGUUG- -5' |
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26485 | 3' | -54.6 | NC_005357.1 | + | 41819 | 0.69 | 0.464629 |
Target: 5'- uCCACG-GCGCu-GCUGGCCgacccgcagGCGGCc -3' miRNA: 3'- cGGUGCaCGCGcuUGACCGGa--------UGUUG- -5' |
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26485 | 3' | -54.6 | NC_005357.1 | + | 41966 | 0.69 | 0.496019 |
Target: 5'- aCCGCGacaGCGCccgcaAGCUGGCCgcGCAGCa -3' miRNA: 3'- cGGUGCa--CGCGc----UUGACCGGa-UGUUG- -5' |
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26485 | 3' | -54.6 | NC_005357.1 | + | 42022 | 0.66 | 0.640211 |
Target: 5'- cGCgACGUGCugcucaacagucGCGcGCUGGCCgcgcuCAAUc -3' miRNA: 3'- -CGgUGCACG------------CGCuUGACCGGau---GUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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