Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26485 | 3' | -54.6 | NC_005357.1 | + | 2575 | 0.69 | 0.484394 |
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Target: 5'- gGCCcagcGCGUGCGCGcGCUuGCCgcccuguUGCAACc -3' miRNA: 3'- -CGG----UGCACGCGCuUGAcCGG-------AUGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 12214 | 0.69 | 0.464629 |
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Target: 5'- cGCCgaguGCGUGCGCGuGCUcaaGCCcgGCGGCu -3' miRNA: 3'- -CGG----UGCACGCGCuUGAc--CGGa-UGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 41819 | 0.69 | 0.464629 |
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Target: 5'- uCCACG-GCGCu-GCUGGCCgacccgcagGCGGCc -3' miRNA: 3'- cGGUGCaCGCGcuUGACCGGa--------UGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 29942 | 0.69 | 0.454397 |
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Target: 5'- cGCCACuGUGCGCG-GCUGGUUcuCGAa -3' miRNA: 3'- -CGGUG-CACGCGCuUGACCGGauGUUg -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 30418 | 0.71 | 0.350722 |
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Target: 5'- gGCCACGgccGCGCu-GCUGGCCgACGu- -3' miRNA: 3'- -CGGUGCa--CGCGcuUGACCGGaUGUug -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 26379 | 1.11 | 0.000544 |
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Target: 5'- gGCCACGUGCGCGAACUGGCCUACAACu -3' miRNA: 3'- -CGGUGCACGCGCUUGACCGGAUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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