Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26485 | 3' | -54.6 | NC_005357.1 | + | 39600 | 0.66 | 0.640211 |
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Target: 5'- cGCCgcugcGCGUGgcCGCGAGCaccUGGCCcgACGAa -3' miRNA: 3'- -CGG-----UGCAC--GCGCUUG---ACCGGa-UGUUg -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 26333 | 0.67 | 0.628892 |
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Target: 5'- -aCGCGcacCGCGAACUGGCCgcGCcGCu -3' miRNA: 3'- cgGUGCac-GCGCUUGACCGGa-UGuUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 39492 | 0.67 | 0.583749 |
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Target: 5'- uGCCGCGcGaCGCGGugUgGGCCgGCAu- -3' miRNA: 3'- -CGGUGCaC-GCGCUugA-CCGGaUGUug -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 38593 | 0.68 | 0.561394 |
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Target: 5'- cGCaaaGUGCGCuGGAUUGGUC-GCAACg -3' miRNA: 3'- -CGgugCACGCG-CUUGACCGGaUGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 23291 | 0.68 | 0.561394 |
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Target: 5'- cGCCACGaaugccuguuUGCGgcCGGcACUGGCCgggAUGACg -3' miRNA: 3'- -CGGUGC----------ACGC--GCU-UGACCGGa--UGUUG- -5' |
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| 26485 | 3' | -54.6 | NC_005357.1 | + | 37111 | 0.76 | 0.197059 |
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Target: 5'- cGCCACGUGCuCGc---GGCCUACGGCc -3' miRNA: 3'- -CGGUGCACGcGCuugaCCGGAUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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