Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26485 | 5' | -56.1 | NC_005357.1 | + | 8523 | 0.7 | 0.42796 |
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Target: 5'- -----aUGUCGCCGGCGAaCUUG-CCCa -3' miRNA: 3'- gccaaaGCAGUGGCCGCU-GGACaGGG- -5' |
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| 26485 | 5' | -56.1 | NC_005357.1 | + | 25912 | 0.7 | 0.399966 |
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Target: 5'- gGGUUUCGacuugcagcUugCGGUGGCCcagcaUGUCCCg -3' miRNA: 3'- gCCAAAGCa--------GugGCCGCUGG-----ACAGGG- -5' |
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| 26485 | 5' | -56.1 | NC_005357.1 | + | 37596 | 0.7 | 0.381965 |
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Target: 5'- uGGUcaUCGacuaCGGCGACCUGUCgCCg -3' miRNA: 3'- gCCAa-AGCagugGCCGCUGGACAG-GG- -5' |
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| 26485 | 5' | -56.1 | NC_005357.1 | + | 30988 | 0.71 | 0.373169 |
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Target: 5'- cCGGUgUCGcgccUUAUCGGCGACCUGcacgCCg -3' miRNA: 3'- -GCCAaAGC----AGUGGCCGCUGGACa---GGg -5' |
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| 26485 | 5' | -56.1 | NC_005357.1 | + | 30595 | 0.72 | 0.323342 |
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Target: 5'- uGGgauacgUCGguggCAUCGGCGAUCUGUUCUu -3' miRNA: 3'- gCCaa----AGCa---GUGGCCGCUGGACAGGG- -5' |
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| 26485 | 5' | -56.1 | NC_005357.1 | + | 37110 | 0.72 | 0.315536 |
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Target: 5'- uGGUaucCGUgGCCGGCGACCUGg--- -3' miRNA: 3'- gCCAaa-GCAgUGGCCGCUGGACaggg -5' |
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| 26485 | 5' | -56.1 | NC_005357.1 | + | 26416 | 1.12 | 0.00045 |
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Target: 5'- aCGGUUUCGUCACCGGCGACCUGUCCCu -3' miRNA: 3'- -GCCAAAGCAGUGGCCGCUGGACAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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