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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2649 | 3' | -58.9 | NC_001491.2 | + | 149733 | 0.66 | 0.769767 |
Target: 5'- -cGGAGuGG-GCGUGgCCUCccaGUGGGc -3' miRNA: 3'- gaCCUC-CCaCGCACgGGAGac-UACCC- -5' |
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2649 | 3' | -58.9 | NC_001491.2 | + | 130664 | 0.7 | 0.583182 |
Target: 5'- uUGGGGGGUGcCGguagcgGCCCUUg---GGGa -3' miRNA: 3'- gACCUCCCAC-GCa-----CGGGAGacuaCCC- -5' |
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2649 | 3' | -58.9 | NC_001491.2 | + | 108492 | 1.09 | 0.001528 |
Target: 5'- cCUGGAGGGUGCGUGCCCUCUGAUGGGc -3' miRNA: 3'- -GACCUCCCACGCACGGGAGACUACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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