Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 26492 | 3' | -59.7 | NC_005357.1 | + | 40183 | 0.67 | 0.336244 |
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Target: 5'- gUCGAacuGCCggCCAAGGCCCGGCguuuauaccgCGAcaugGAGCg -3' miRNA: 3'- -AGUU---CGG--GGUUCCGGGCCG----------GCU----CUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 38032 | 0.67 | 0.336244 |
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Target: 5'- gCAcG-CUCAAGGCCCGGCgCGAcGGCg -3' miRNA: 3'- aGUuCgGGGUUCCGGGCCG-GCUcUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 29670 | 0.66 | 0.393816 |
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Target: 5'- ---uGCCCagCAGGGCgCGGCCGuguguuugaaaaucGGAACu -3' miRNA: 3'- aguuCGGG--GUUCCGgGCCGGC--------------UCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 37816 | 0.66 | 0.378616 |
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Target: 5'- gCGGGCCaacauuGGCCUGGCUGAccuGGGCg -3' miRNA: 3'- aGUUCGGgguu--CCGGGCCGGCU---CUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 13056 | 0.66 | 0.404781 |
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Target: 5'- cCGAGUCCgAGGGCuggggcaaggccaCCGGCgcgugguuCGAGGACu -3' miRNA: 3'- aGUUCGGGgUUCCG-------------GGCCG--------GCUCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 39578 | 0.65 | 0.412198 |
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Target: 5'- cCAGGCCCCucgcuGGuGCUggcgccgcugcgcgUGGCCGcGAGCa -3' miRNA: 3'- aGUUCGGGGu----UC-CGG--------------GCCGGCuCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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