Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26492 | 3' | -59.7 | NC_005357.1 | + | 13056 | 0.66 | 0.404781 |
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Target: 5'- cCGAGUCCgAGGGCuggggcaaggccaCCGGCgcgugguuCGAGGACu -3' miRNA: 3'- aGUUCGGGgUUCCG-------------GGCCG--------GCUCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 12804 | 0.71 | 0.171896 |
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Target: 5'- aUCAAGCCCggccaCAAGcGCCCGGCgcuGGACa -3' miRNA: 3'- -AGUUCGGG-----GUUC-CGGGCCGgcuCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 9300 | 0.67 | 0.339504 |
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Target: 5'- aCAAGCCCgaucacggcgacauuCAGGGCCUuggguucauGGgCGAGGAUg -3' miRNA: 3'- aGUUCGGG---------------GUUCCGGG---------CCgGCUCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 6339 | 0.68 | 0.297438 |
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Target: 5'- ---cGCCCCAGccaaagcgcccGGCCCgaaGGCCGGGGc- -3' miRNA: 3'- aguuCGGGGUU-----------CCGGG---CCGGCUCUug -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 1166 | 0.68 | 0.307942 |
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Target: 5'- gUCAcGCCCCAcauagucucgccgccGGGgUCGGCCGGGu-- -3' miRNA: 3'- -AGUuCGGGGU---------------UCCgGGCCGGCUCuug -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 676 | 0.69 | 0.262197 |
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Target: 5'- cCAGGCCgCCuacggcGGCgCCGGCCGGGc-- -3' miRNA: 3'- aGUUCGG-GGuu----CCG-GGCCGGCUCuug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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