Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26492 | 3' | -59.7 | NC_005357.1 | + | 27249 | 0.7 | 0.224478 |
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Target: 5'- cCGGcGCCCC-AGGCUCuuGGCCGAGGc- -3' miRNA: 3'- aGUU-CGGGGuUCCGGG--CCGGCUCUug -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 32367 | 0.7 | 0.212969 |
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Target: 5'- ---cGCCgCCGAGGcCCCGGCCGcccAGCa -3' miRNA: 3'- aguuCGG-GGUUCC-GGGCCGGCuc-UUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 23740 | 0.7 | 0.201433 |
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Target: 5'- ---uGCUgaCCGAGGCCCgcgugguguaacaGGCCGGGGGCg -3' miRNA: 3'- aguuCGG--GGUUCCGGG-------------CCGGCUCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 12804 | 0.71 | 0.171896 |
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Target: 5'- aUCAAGCCCggccaCAAGcGCCCGGCgcuGGACa -3' miRNA: 3'- -AGUUCGGG-----GUUC-CGGGCCGgcuCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 22201 | 0.74 | 0.110412 |
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Target: 5'- -aAGGCaCCCAAGGCcaacagCCGGCCGcGGGCg -3' miRNA: 3'- agUUCG-GGGUUCCG------GGCCGGCuCUUG- -5' |
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| 26492 | 3' | -59.7 | NC_005357.1 | + | 25327 | 1.08 | 0.000257 |
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Target: 5'- gUCAAGCCCCAAGGCCCGGCCGAGAACc -3' miRNA: 3'- -AGUUCGGGGUUCCGGGCCGGCUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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