Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26496 | 3' | -62.4 | NC_005357.1 | + | 8578 | 0.66 | 0.302229 |
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Target: 5'- cGUCguagGCCGCGcGUGCGGCCUgguGCUGGa -3' miRNA: 3'- -CAG----UGGCGCuCGCGCCGGAgg-UGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 7342 | 0.73 | 0.09021 |
|
Target: 5'- aUgGCCuuGAGCGCGGCCUCgACCu- -3' miRNA: 3'- cAgUGGcgCUCGCGCCGGAGgUGGcu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 6077 | 0.68 | 0.224248 |
|
Target: 5'- -aCACCGCcucgaagucgucGAGCGCGGCCUUgCGCa-- -3' miRNA: 3'- caGUGGCG------------CUCGCGCCGGAG-GUGgcu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 5349 | 0.66 | 0.295 |
|
Target: 5'- -gCAUCGCGcuGCGUGGCCgCCugCa- -3' miRNA: 3'- caGUGGCGCu-CGCGCCGGaGGugGcu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 3875 | 0.68 | 0.230034 |
|
Target: 5'- cGUCGCUGUaGGccGCGCuGCCgucgCCGCCGAa -3' miRNA: 3'- -CAGUGGCG-CU--CGCGcCGGa---GGUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 2944 | 0.66 | 0.302229 |
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Target: 5'- -aCACCGCGucGCGCGGCa--CGUCGAg -3' miRNA: 3'- caGUGGCGCu-CGCGCCGgagGUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 1706 | 0.69 | 0.202329 |
|
Target: 5'- uUCGCCGUGGGC-CGGCgUgCCGgCGAg -3' miRNA: 3'- cAGUGGCGCUCGcGCCGgA-GGUgGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 1303 | 0.69 | 0.197147 |
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Target: 5'- -cCGCCGCGcccagGGCGaCGGCCaCCagGCCGAc -3' miRNA: 3'- caGUGGCGC-----UCGC-GCCGGaGG--UGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 384 | 0.69 | 0.187132 |
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Target: 5'- --uGCCGauuGAGCGCGGCCagCGCgCGAc -3' miRNA: 3'- cagUGGCg--CUCGCGCCGGagGUG-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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