Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26496 | 3' | -62.4 | NC_005357.1 | + | 384 | 0.69 | 0.187132 |
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Target: 5'- --uGCCGauuGAGCGCGGCCagCGCgCGAc -3' miRNA: 3'- cagUGGCg--CUCGCGCCGGagGUG-GCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 14590 | 0.69 | 0.192082 |
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Target: 5'- --aGCaGCG-GCGCGGCCggcgCCACCGc -3' miRNA: 3'- cagUGgCGCuCGCGCCGGa---GGUGGCu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 14917 | 0.69 | 0.192082 |
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Target: 5'- --gACCGCGccgagaAGCugGCGGCCgaggCCGCCGGc -3' miRNA: 3'- cagUGGCGC------UCG--CGCCGGa---GGUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 29420 | 0.68 | 0.207628 |
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Target: 5'- -cCACUGCGcuGCGCGGCCUgUACgaGAa -3' miRNA: 3'- caGUGGCGCu-CGCGCCGGAgGUGg-CU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 22223 | 0.68 | 0.207628 |
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Target: 5'- --gGCCGCGGGCGCuGCCaagACCGAg -3' miRNA: 3'- cagUGGCGCUCGCGcCGGaggUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 22934 | 0.68 | 0.224248 |
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Target: 5'- -cCACgGUucGCaGCGGCCUgCCACCGGc -3' miRNA: 3'- caGUGgCGcuCG-CGCCGGA-GGUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 6077 | 0.68 | 0.224248 |
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Target: 5'- -aCACCGCcucgaagucgucGAGCGCGGCCUUgCGCa-- -3' miRNA: 3'- caGUGGCG------------CUCGCGCCGGAG-GUGgcu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 16647 | 0.68 | 0.224248 |
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Target: 5'- -gCGCCGCGcGCaucGCGGCCaucguugCCGCCGc -3' miRNA: 3'- caGUGGCGCuCG---CGCCGGa------GGUGGCu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 23862 | 1.08 | 0.000188 |
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Target: 5'- cGUCACCGCGAGCGCGGCCUCCACCGAc -3' miRNA: 3'- -CAGUGGCGCUCGCGCCGGAGGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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