Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26496 | 3' | -62.4 | NC_005357.1 | + | 33347 | 0.66 | 0.302229 |
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Target: 5'- -cCACCGCuGGCuaucGCGGCacggCCACCGc -3' miRNA: 3'- caGUGGCGcUCG----CGCCGga--GGUGGCu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 33251 | 0.66 | 0.302229 |
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Target: 5'- -cCACCGCuGGCuaucGCGGCacggCCACCGc -3' miRNA: 3'- caGUGGCGcUCG----CGCCGga--GGUGGCu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 33203 | 0.66 | 0.302229 |
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Target: 5'- -cCACCGCuGGCuaucGCGGCacggCCACCGc -3' miRNA: 3'- caGUGGCGcUCG----CGCCGga--GGUGGCu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 10343 | 0.66 | 0.302229 |
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Target: 5'- cGUCGCUGCG-GCGUGuGCCUUCuucuCCc- -3' miRNA: 3'- -CAGUGGCGCuCGCGC-CGGAGGu---GGcu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 17067 | 0.66 | 0.297876 |
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Target: 5'- uUCACCucGCGcacgaaggcgaagccGGCGCGGuUCUCgGCCGGg -3' miRNA: 3'- cAGUGG--CGC---------------UCGCGCC-GGAGgUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 38471 | 0.66 | 0.295 |
|
Target: 5'- -cUACCGCG-GCuaCGGUUUCCGCCGu -3' miRNA: 3'- caGUGGCGCuCGc-GCCGGAGGUGGCu -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 10689 | 0.66 | 0.287907 |
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Target: 5'- cGUCuugACgGC-AGCGCgcacGGCCUCgGCCGAu -3' miRNA: 3'- -CAG---UGgCGcUCGCG----CCGGAGgUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 18064 | 0.66 | 0.280948 |
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Target: 5'- -cCGgCGCgGGGUGCGuGCUgggCCGCCGAa -3' miRNA: 3'- caGUgGCG-CUCGCGC-CGGa--GGUGGCU- -5' |
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| 26496 | 3' | -62.4 | NC_005357.1 | + | 24736 | 0.79 | 0.036619 |
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Target: 5'- -cCACCGCGccuGCGCGGCCUCgACCc- -3' miRNA: 3'- caGUGGCGCu--CGCGCCGGAGgUGGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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