Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 265 | 3' | -54.5 | AC_000008.1 | + | 19015 | 0.66 | 0.656782 |
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Target: 5'- aCGCUGCGGU-UCAUCccuguGGACCGUGa- -3' miRNA: 3'- -GUGGCGCCGcAGUAGc----UUUGGCACau -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 23130 | 0.66 | 0.656782 |
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Target: 5'- -cCCGCGGCa-CGUUGGcgGGCUGUGUAc -3' miRNA: 3'- guGGCGCCGcaGUAGCU--UUGGCACAU- -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 16879 | 0.66 | 0.645381 |
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Target: 5'- aACCGCaGCGUCAaacgcUGGGACCGgucUGUGg -3' miRNA: 3'- gUGGCGcCGCAGUa----GCUUUGGC---ACAU- -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 17051 | 0.67 | 0.588401 |
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Target: 5'- cCACUGCGGCaUCAUCGAAgggguaGCCa---- -3' miRNA: 3'- -GUGGCGCCGcAGUAGCUU------UGGcacau -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 26458 | 0.67 | 0.565814 |
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Target: 5'- cCGgUGCGcCGUCGUCGccGCCGUGUc -3' miRNA: 3'- -GUgGCGCcGCAGUAGCuuUGGCACAu -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 26389 | 0.67 | 0.565814 |
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Target: 5'- gGCCGCGcCGUCAcCGAGACCa---- -3' miRNA: 3'- gUGGCGCcGCAGUaGCUUUGGcacau -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 19850 | 0.7 | 0.398165 |
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Target: 5'- cCACCGCgucgauGGCGUCAUCGA--CgGUGg- -3' miRNA: 3'- -GUGGCG------CCGCAGUAGCUuuGgCACau -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 17839 | 0.71 | 0.352599 |
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Target: 5'- uCGCCGCGGCG--AUUGgcGCCGUGc- -3' miRNA: 3'- -GUGGCGCCGCagUAGCuuUGGCACau -5' |
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| 265 | 3' | -54.5 | AC_000008.1 | + | 21613 | 1.06 | 0.001123 |
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Target: 5'- gCACCGCGGCGUCAUCGAAACCGUGUAc -3' miRNA: 3'- -GUGGCGCCGCAGUAGCUUUGGCACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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