Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2650 | 3' | -53 | NC_001491.2 | + | 2084 | 0.66 | 0.961876 |
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Target: 5'- gCCuGGCGGagcccagGCGCaggCAGAGGUa-- -3' miRNA: 3'- gGGuUCGCCa------CGCGaa-GUCUCCAaaa -5' |
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| 2650 | 3' | -53 | NC_001491.2 | + | 4823 | 0.67 | 0.936053 |
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Target: 5'- gCCgGAGCgGGUGCGC--CGGGGGa--- -3' miRNA: 3'- -GGgUUCG-CCACGCGaaGUCUCCaaaa -5' |
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| 2650 | 3' | -53 | NC_001491.2 | + | 6997 | 0.68 | 0.901171 |
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Target: 5'- gCCAGGCGGUgGCGCUguuUCAaaacacGGUUUUa -3' miRNA: 3'- gGGUUCGCCA-CGCGA---AGUcu----CCAAAA- -5' |
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| 2650 | 3' | -53 | NC_001491.2 | + | 34771 | 0.69 | 0.872946 |
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Target: 5'- gCCCGGGaCGGcgcGCGCUUCGGGGa---- -3' miRNA: 3'- -GGGUUC-GCCa--CGCGAAGUCUCcaaaa -5' |
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| 2650 | 3' | -53 | NC_001491.2 | + | 105338 | 0.69 | 0.865302 |
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Target: 5'- uCCaCAGGgGGUGCGUcuUUUGGGGGUg-- -3' miRNA: 3'- -GG-GUUCgCCACGCG--AAGUCUCCAaaa -5' |
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| 2650 | 3' | -53 | NC_001491.2 | + | 3431 | 0.72 | 0.718052 |
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Target: 5'- gCCCAGGCGGUGgGUagggUCAGAGu---- -3' miRNA: 3'- -GGGUUCGCCACgCGa---AGUCUCcaaaa -5' |
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| 2650 | 3' | -53 | NC_001491.2 | + | 102555 | 1.07 | 0.006576 |
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Target: 5'- gCCCAAGCGGUGCGCUUCAGAGGUUUUu -3' miRNA: 3'- -GGGUUCGCCACGCGAAGUCUCCAAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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