Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26502 | 3' | -50.6 | NC_005357.1 | + | 15058 | 0.71 | 0.572124 |
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Target: 5'- --uGCUGGccgaaugGCUgGACGAG-UGCUGCg -3' miRNA: 3'- cguUGACCa------CGAgCUGUUCuACGACG- -5' |
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| 26502 | 3' | -50.6 | NC_005357.1 | + | 31326 | 0.72 | 0.526632 |
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Target: 5'- cCAAUUGGccgcaGC-CGACAAGGUGCUcGCg -3' miRNA: 3'- cGUUGACCa----CGaGCUGUUCUACGA-CG- -5' |
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| 26502 | 3' | -50.6 | NC_005357.1 | + | 32853 | 0.74 | 0.450711 |
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Target: 5'- cGCAAC--GUGUUCGACcGGAUGCUcGCc -3' miRNA: 3'- -CGUUGacCACGAGCUGuUCUACGA-CG- -5' |
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| 26502 | 3' | -50.6 | NC_005357.1 | + | 8594 | 0.77 | 0.279763 |
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Target: 5'- uGCGGcCUGGUGCUgGAUAAGcugGUUGCg -3' miRNA: 3'- -CGUU-GACCACGAgCUGUUCua-CGACG- -5' |
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| 26502 | 3' | -50.6 | NC_005357.1 | + | 32525 | 0.78 | 0.244291 |
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Target: 5'- uGCAgGCUGGUGaugcCGGcCAGGAUGCUGCc -3' miRNA: 3'- -CGU-UGACCACga--GCU-GUUCUACGACG- -5' |
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| 26502 | 3' | -50.6 | NC_005357.1 | + | 22384 | 1.14 | 0.000877 |
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Target: 5'- cGCAACUGGUGCUCGACAAGAUGCUGCc -3' miRNA: 3'- -CGUUGACCACGAGCUGUUCUACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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