miRNA: 3'- -GGUGCGCCAguagGUGGACCuGCacu -5'
miRNA: 3'- -GGUGCGCCAGUaGgU-GGACCUGCa-CU- -5'
miRNA: 3'- ggUGCGCCA-GUa---GGUGG--ACCUGCaCU- -5'
miRNA: 3'- -GGUGCGCCa------GUAGgUGG----------ACCugCACU- -5'
miRNA: 3'- gGUGC-GCCAguaGGUGG--ACCUGCAcu -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26514 | 3' | -56.1 | NC_005357.1 | + | 28122 | 0.67 | 0.581922 |
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Target: 5'- gUCACGCGGUa---CGCCUGGuCGg-- -3' miRNA: 3'- -GGUGCGCCAguagGUGGACCuGCacu -5' |
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| 26514 | 3' | -56.1 | NC_005357.1 | + | 25739 | 0.66 | 0.614811 |
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Target: 5'- gCUACGUGGUCA-CgGcCCUGGAUGccGAg -3' miRNA: 3'- -GGUGCGCCAGUaGgU-GGACCUGCa-CU- -5' |
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| 26514 | 3' | -56.1 | NC_005357.1 | + | 21724 | 0.66 | 0.636827 |
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Target: 5'- -aACGCGGUaCGgaugCCACCguUGGGCGcGGc -3' miRNA: 3'- ggUGCGCCA-GUa---GGUGG--ACCUGCaCU- -5' |
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| 26514 | 3' | -56.1 | NC_005357.1 | + | 19627 | 0.66 | 0.647833 |
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Target: 5'- gUCAUGCGGgccgcgaCAUCgGCCagcgucggguUGGugGUGGa -3' miRNA: 3'- -GGUGCGCCa------GUAGgUGG----------ACCugCACU- -5' |
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| 26514 | 3' | -56.1 | NC_005357.1 | + | 3752 | 0.66 | 0.647833 |
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Target: 5'- gCACGuCGGUg--CCGCCgcUGGACGUa- -3' miRNA: 3'- gGUGC-GCCAguaGGUGG--ACCUGCAcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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