Results 81 - 83 of 83 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26515 | 5' | -60.8 | NC_005357.1 | + | 41791 | 0.67 | 0.345665 |
Target: 5'- gGGCAccGACucgACGCCGGACGccguguCCaCGGCg -3' miRNA: 3'- -CCGUucCUG---UGCGGCCUGCc-----GG-GCUG- -5' |
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26515 | 5' | -60.8 | NC_005357.1 | + | 42115 | 0.67 | 0.33763 |
Target: 5'- cGGUAcGG-CACGCCcuGGACGGaagagcgcgccuUCCGGCg -3' miRNA: 3'- -CCGUuCCuGUGCGG--CCUGCC------------GGGCUG- -5' |
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26515 | 5' | -60.8 | NC_005357.1 | + | 42245 | 0.69 | 0.258273 |
Target: 5'- uGGCGGGGAUgACGCCGGcauucugcgcGCGGCagcuugGGCa -3' miRNA: 3'- -CCGUUCCUG-UGCGGCC----------UGCCGgg----CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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