miRNA: 3'- -CCGUUc----CUGu-GCGGCCUGC---CGGGCUG- -5'
miRNA: 3'- -CCGU----UCC---UGUGCGGCC-----------UG-CCGGGCUG- -5'
miRNA: 3'- -CCGUUCCUGUGCGGCCUGCCGGGCUG- -5'
Results 81 - 83 of 83 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26515 | 5' | -60.8 | NC_005357.1 | + | 18475 | 0.7 | 0.221793 |
|
Target: 5'- cGGCAAaugccGACcuCGCCGGGCGugcGCCUGACc -3' miRNA: 3'- -CCGUUc----CUGu-GCGGCCUGC---CGGGCUG- -5' |
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| 26515 | 5' | -60.8 | NC_005357.1 | + | 33576 | 0.7 | 0.221793 |
|
Target: 5'- uGGCGaccgAGGccaACGCGCUGGcccgcguugcgGCuGGCCCGACc -3' miRNA: 3'- -CCGU----UCC---UGUGCGGCC-----------UG-CCGGGCUG- -5' |
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| 26515 | 5' | -60.8 | NC_005357.1 | + | 17840 | 1.1 | 0.000191 |
|
Target: 5'- cGGCAAGGACACGCCGGACGGCCCGACc -3' miRNA: 3'- -CCGUUCCUGUGCGGCCUGCCGGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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