miRNA: 3'- -UCCGCGCG------CUGCUGaUGUUC---UCGCUG- -5'
miRNA: 3'- uCCGCGCGCUGc--UGAUGUUC---UCGCUG- -5'
miRNA: 3'- -UCCGCGCGCUGCUGAUGUUCUCGCUG- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26519 | 5' | -56.1 | NC_005357.1 | + | 34378 | 0.77 | 0.135602 |
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Target: 5'- uGGGCGCGCgucaugGACGGCgGCAAGgacGGCGGCc -3' miRNA: 3'- -UCCGCGCG------CUGCUGaUGUUC---UCGCUG- -5' |
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| 26519 | 5' | -56.1 | NC_005357.1 | + | 31237 | 0.83 | 0.043552 |
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Target: 5'- cGGCGCGCGGCccaGCUACGAGccgGGCGACg -3' miRNA: 3'- uCCGCGCGCUGc--UGAUGUUC---UCGCUG- -5' |
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| 26519 | 5' | -56.1 | NC_005357.1 | + | 15024 | 1.1 | 0.00048 |
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Target: 5'- cAGGCGCGCGACGACUACAAGAGCGACa -3' miRNA: 3'- -UCCGCGCGCUGCUGAUGUUCUCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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