Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26519 | 5' | -56.1 | NC_005357.1 | + | 33731 | 0.67 | 0.539575 |
Target: 5'- cGGCagcaaucuGCGCGGCGcAC-ACcuGGGCGGCg -3' miRNA: 3'- uCCG--------CGCGCUGC-UGaUGuuCUCGCUG- -5' |
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26519 | 5' | -56.1 | NC_005357.1 | + | 16470 | 0.67 | 0.539575 |
Target: 5'- cGGGgGCGUGACGGacagguCGGGcGCGAUa -3' miRNA: 3'- -UCCgCGCGCUGCUgau---GUUCuCGCUG- -5' |
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26519 | 5' | -56.1 | NC_005357.1 | + | 31237 | 0.83 | 0.043552 |
Target: 5'- cGGCGCGCGGCccaGCUACGAGccgGGCGACg -3' miRNA: 3'- uCCGCGCGCUGc--UGAUGUUC---UCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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