Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2652 | 5' | -64.7 | NC_001491.2 | + | 26758 | 0.66 | 0.501784 |
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Target: 5'- cGGGCucGGcgCCCGCCauguugaguagcGCgGCCCaguaGCGCu -3' miRNA: 3'- aCCCGu-CCa-GGGCGG------------CGgUGGG----UGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 33917 | 0.66 | 0.501784 |
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Target: 5'- cGGGCcucGG-CCUGgaCGUCACCCAgGCc -3' miRNA: 3'- aCCCGu--CCaGGGCg-GCGGUGGGUgCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 90299 | 0.66 | 0.4918 |
|
Target: 5'- aUGGGUGGGgucacacagcUCCggggguaccacuaUGCCGCUGCCCACa- -3' miRNA: 3'- -ACCCGUCC----------AGG-------------GCGGCGGUGGGUGcg -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 64460 | 0.66 | 0.4918 |
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Target: 5'- aGGuuucGCAGGUCCCGUagugcuuucgUGCCacuagggagaucgGCCUGCGCc -3' miRNA: 3'- aCC----CGUCCAGGGCG----------GCGG-------------UGGGUGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 84768 | 0.65 | 0.547252 |
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Target: 5'- cGGGCagugccaAGGUCguaGCCGCUucuaccGCCC-CGCa -3' miRNA: 3'- aCCCG-------UCCAGgg-CGGCGG------UGGGuGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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